academyzuloo.blogg.se - Canine minitube

Canine minitube full#
Canine minitube plus#

As such we believe our people safe and importantly most of our people feel so. We have had no case of contamination within Minitubes premise.

We have put in place a set of rules and have adapted working station to insure social distancing.

On top of that we have spent the past few months preparing for the eventuality of a second lock down: This may impact our efficiencies on some project work, but not on our production capacity.

People who can work remotely are extremely encouraged to do so.

We won’t face this issue this time around. During the first lockdown we suffered from a high absenteeism of our staff with kids.

This lockdown maintain schools opened.

The French President has explicitly mentioned that the industries/factories have to keep running.

This lockdown allows economic activity to keep going.

Let me share a few details on the second French Lockdown and how it sometime differs from the first one: We understand our duty as supplier for the medical industry and have organized ourselves to keep the factory running while assuring the security of our staff. Let me start by reassuring you that Minitubes activity will be maintained in its integrality. Transcervical insemination has the advantage of allowing two inseminations when there are enough straws available to do so.You may have heard that France entered today its second national lockdown. Many factors influence this figure, especially the quality of the individual semen sample and the number of inseminations. Surgical or transcervical insemination yields a success rate as high as 85%. Vaginal deposition generally leads to a success rate <25% because the thawed sperm only live for about 12 hours. Success with frozen semen depends on consistent attention to detail, good breeding management (ovulation timing), and intrauterine deposition of semen by surgical or transcervical insemination.

Canine minitube full#

A tester straw (the last, partially full one) is always thawed and checked in order to make the needed calculations. Most collections yield between one and five breedings. Some dogs’ semen may not perform as well, meaning more straws per breeding.

Canine minitube plus#

In other words, some dogs freeze very well and give 70% return on motility plus minimal defects post-thaw. The number of breedings possible when using a frozen sample depends on the semen evaluation and the dog’s individual sensitivity to the freezing process.

We need 100 million normal, motile sperm per breeding. The number of straws needed for breeding depends on the quality of the semen (motility and morphology). The number of straws produced depends on the total number of sperm per ejaculate. We collect the male, do a semen evaluation, and process the semen to allow about 50 million sperm per straw. For example, if a sample has 80% motility pre-freezing, then we can get 56% progressive motility post-thaw. Using Minitube’s methodology and equipment, we can approach a 70% return of sperm motility on thawing of frozen semen. This progressive company has been processing 95% of the world’s porcine (pig) semen for years, and they are now applying their expertise to canine reproductive techniques. We work with Minitube products and technology. When frozen semen is thawed, the optimal rate is fast enough to prevent ice crystal formation, but slow enough to prevent cells from rupturing as fluid re-enters. The rate of freezing should be fast enough to prevent excessive shrinkage of sperm cells as fluid leaves them (changing osmotic gradient), but slow enough to prevent ice from forming inside the cells. Antibiotics are added to prevent bacterial growth, sugars are included to provide energy, and fresh egg yolk is used to protect cell membranes from cold shock. Buffering compounds maintain an optimum pH of 6.9-7.1 despite hydrogen ion production by sperm metabolic activity (tends to increase the pH). Glycerol, a cryoprotective agent, is allowed to penetrate the cells after chilling. Special extenders are designed to protect the sperm cells during freezing.

Once frozen, semen can be stored indefinitely. We must be meticulous, but we do not require ‘100% clean rooms’ or other high-priced technological aids. Luckily, canine semen is less sensitive than many other species. When we freeze semen in liquid nitrogen, we are asking the sperm cells to survive a drop in temperature from 37 C to 4 C during the chilling process, followed by a plunge to –196 C during the freezing process.